Comparative Study on the Phytochemical Analysis of Costus spicatus (Jacq.) Leaf and Rhizome

Costus spicatus, an herbaceous plant, parts- leaves, rhizome, stem and root are used locally because of its medicinal properties. This study was carried out to compare the phyto- constituents of the Costus spicatus leaf and rhizome. The secondary metabolites of Costus spicatusleaf and rhizomewere determined qualitatively via standard phytochemicalscreening methods.Thechemicalcompositions of the aqueousextract ofCostusspicatusleaf and rhizome were analyzed using Gas Chromatography-Mass Spectrometry and UV-Visible Spectrophotometer. The qualitative phytochemical analysis revealed the presence of Carbohydrate, Alkaloids, Saponins, Terpenoids, Phenols and Protein were detected in the aqueous rhizome extract while Carbohydrate, Alkaloids, Flavonoids and Proteins were detected in the leaf extract. The UV-yis scan of the aqueous rhizome and leaf extracts revealed the varying absorbance of the leaf and rhizome extract at different wavelengths and the spectrum shows the highest peaks at 200-214 nm and 220-227 nm respectively indicating the presence of organic chromophores. The GC-MS analysis of aqueous extract of Costus spicatus rhizome revealed the presence of Hexadecanoic acid, methyl ester (11.34%) and 10,13-Octadecadienoic acid, methylester (10.22%) for the leaf extract as the compound with the highest peaks. These results reveal that the rhizome extract contains more active organic compounds and therefore can be further explored in phytomedicinal researches.